关键词: 电针, 营养性肥胖, 脏器系数, 芳香化酶

Abstract: Objective　To observe the effects of electroacupuncture on organ coefficient and ovarian aromatase (CYP19A1) gene and protein expression induced by high-fat diet in female growing rats. Methods　21-day-old female SD rats were randomly divided into control, model and acupuncture groups, with 6 rats in each group. The control group was fed with regular feed; the model group was fed with high-fat feed without any treatment; the electroacupuncture group was fed with high-fat feed and selected “Sanyinjiao” (SP6), “Fenglong” (ST40) and “Zusanli” (ST36) for electroacupuncture treatment for 14 days. The body mass and length of rats were measured weekly. After treatment, the body mass, liver mass, ovarian mass, and uterine mass of rats were weighed on an electronic balance. The liver coefficient (liver mass/body mass), ovarian coefficient (ovarian mass/body mass), and uterine coefficient (uterus mass/body mass) were calculated. Fluorescence quantitative PCR was used to detect the expression level of CYP19A1 mRNA in rat ovaries, Western blot method for detecting the expression level of CYP19A1 protein in rat ovarian tissue.Results　Compared with the control group, the body mass and Lee’s index of the model group were significantly increased (P<0.05), the organ coefficient was significantly increased (P<0.05), and the expression of ovarian CYP19A1 mRNA and protein was significantly increased (P<0.05). Compared with the model group, the body weight and Lee’s index of the acupuncture group rats were significantly reduced (P<0.05), while the organ coefficient and the expression of ovarian CYP19A1 mRNA and protein were significantly downregulated (P<0.05). Conclusion　Electroacupuncture can reduce the Lee’s index and organ coefficient of high-fat diet induced obese female growth period rats, downregulate the expression levels of ovarian CYP19A1 mRNA and protein, and thus regulate the level of sexual development in female growth period obese rats.

Key words: electroacupuncture, nutritional obesity, organ coefficients, aromatase